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slug sirna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher slug sirna
    (A) Representative images of primary epicardial cells transfected with control <t>siRNA</t> (siControl) or siRNA directed against <t>Tbx18</t> <t>(siTbx18)</t> or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.
    Slug Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slug+sirna/pmc03585213-52-19-23
    Average 90 stars, based on 1 article reviews
    slug sirna - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells"

    Article Title: The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0057829

    (A) Representative images of primary epicardial cells transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18) or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.
    Figure Legend Snippet: (A) Representative images of primary epicardial cells transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18) or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.

    Techniques Used: Transfection, Immunostaining, Staining, Migration, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    (A) Representative images of epicardial cells treated with TGFβ1 and transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18), Slug (siSlug) or Wt1 (siWt1). (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis in TGFβ1-treated epicardial cells transfected with siControl, siTbx18, siSlug or siWt1 (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the TGFβ1+siControl. (E) Western blot performed with antibodies against Tbx18, Wt1, Snail and Slug. β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD. Scale bars: 100 µm.
    Figure Legend Snippet: (A) Representative images of epicardial cells treated with TGFβ1 and transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18), Slug (siSlug) or Wt1 (siWt1). (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis in TGFβ1-treated epicardial cells transfected with siControl, siTbx18, siSlug or siWt1 (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the TGFβ1+siControl. (E) Western blot performed with antibodies against Tbx18, Wt1, Snail and Slug. β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Techniques Used: Transfection, Immunostaining, Staining, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    (A) Representative images of Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug. Epicardial cells transfected with siControl or siWt1 were used as controls. (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNA. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 and Slug by real-time PCR in Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug, in comparison to siControl-transfected epicardial cells (n = 3; * P <0.05 vs. siControl, † P <0.05 vs. siWt1). The results were normalized to Gapdh expression and the relative expression level is provided as a ratio to the siControl. The data are presented as the mean ± SD. Scale bars: 100 µm.
    Figure Legend Snippet: (A) Representative images of Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug. Epicardial cells transfected with siControl or siWt1 were used as controls. (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNA. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 and Slug by real-time PCR in Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug, in comparison to siControl-transfected epicardial cells (n = 3; * P <0.05 vs. siControl, † P <0.05 vs. siWt1). The results were normalized to Gapdh expression and the relative expression level is provided as a ratio to the siControl. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Techniques Used: Transfection, Immunostaining, Staining, Expressing, Real-time Polymerase Chain Reaction

    Related Articles

    Transfection:

    Article Title: MiRNA-203 suppresses tumor cell proliferation, migration and invasion by targeting Slug in gastric cancer
    Article Snippet: .. The Slug siRNA were transfected into MKN-45 cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: The CaSm (LSm1) oncogene promotes transformation, chemoresistance and metastasis of pancreatic cancer cells
    Article Snippet: .. Tet-on CaSm cells were either left untreated or transiently transfected with 5 μg scramble (Stealth RNAi Negative Control, Invitrogen) or Slug siRNA (Invitrogen HSS185949) using Lipofectamine RNAiMAX (Invitrogen). ..

    Article Title: MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer
    Article Snippet: The ready-to-use MMP1 siRNA was purchased from Santa Cruz (sc-41552), while Slug siRNA ( 5’-GCUACCCAAUGGCCUCUCU-3’ ) [ ] were chemically synthesized by Ribobio (Guangzhou, China). .. MCF-7/ADR cells were transfected with 40 nM MMP1 siRNA or Slug siRNA using Lipofectamine 2000 (Invitrogen). .. Total RNA in the cell samples were extracted using the Trizol Reagent (Invitrogen) and then was reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA) according to the manufacturer's instructions.

    Article Title: MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer.
    Article Snippet: The ready-to-use MMP1 siRNA was purchased from Santa Cruz (sc-41552), while Slug siRNA (5’-GCUACCCAAUGGCCUCUCU-3’) [17] were chemically synthesized by Ribobio (Guangzhou, China). .. MCF-7/ADR cells were transfected with 40 nM MMP1 siRNA or Slug siRNA using Lipofectamine 2000 (Invitrogen). .. QRT-PCR analysis Total RNA in the cell samples were extracted using the Trizol Reagent (Invitrogen) and then was reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Negative Control:

    Article Title: The CaSm (LSm1) oncogene promotes transformation, chemoresistance and metastasis of pancreatic cancer cells
    Article Snippet: .. Tet-on CaSm cells were either left untreated or transiently transfected with 5 μg scramble (Stealth RNAi Negative Control, Invitrogen) or Slug siRNA (Invitrogen HSS185949) using Lipofectamine RNAiMAX (Invitrogen). ..

    Control:

    Article Title: The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells
    Article Snippet: For knockdown experiments, stealth siRNA (Invitrogen) or control siRNA (Sigma-Aldrich) was transfected using RNAiMax (Invitrogen) on the day following heart removal. .. The following siRNAs were used: Tbx18 siRNA (siTbx18; MSS233533, siTbx18-2; MSS233532, Invitrogen), Wt1 siRNA (siWt1; MSS212627, siWt1-2; MSS212628, Invitrogen), Slug siRNA (siSlug; MSS237944, Invitrogen) and control siRNA (siControl; SIC-001, Sigma-Aldrich). ..



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    (A) Representative images of primary epicardial cells transfected with control <t>siRNA</t> (siControl) or siRNA directed against <t>Tbx18</t> <t>(siTbx18)</t> or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.
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    Image Search Results


    SNAI1 and SNAI2 inhibited miR-92b expression (A) The expression of miR-92b-3p in HK2 cells treated with TGF-β for 12 h, as detected by qPCR, n = 5. (B) Sequence logo of transcription factor SNAI1 and SNAI2. (C–E) Protein levels of SNAI1, SNAI2, and β-actin in HK2 cells treated with SNAI1 siRNA (siSNAI1), SNAI2 siRNA (siSNAI2), or control siRNA as indicated in the figure, as detected by western blotting; quantitative results are shown; n = 3. Expression of Col1a1 and miR-92b-3p as determined by qPCR; n = 5. (F) Schematic representation of the human THBS3-AS1 promoters with the putative E2-box promoter shown as a red box. (G) A series of truncated THBS3-AS1 promoters fused to the luciferase reporter gene was co-transfected into HEK293T cells under TGF-β stimulation together with the pcDNA3.1 Renilla plasmid (Ctrl). The relative luciferase activity was presented as folds of Ctrl (n = 3). (H) DNA fragments containing the flanking region of the E2-box on the THBS3-AS1 promoters were immunoprecipitated with anti-SNAI1 or anti-SNAI2 antibodies. Data are expressed as means ± SD; n = 6 per group; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Similar results from three independent experiments are shown.

    Journal: iScience

    Article Title: MircroRNA-92b as a negative regulator of the TGF-β signaling by targeting the type I receptor

    doi: 10.1016/j.isci.2023.108131

    Figure Lengend Snippet: SNAI1 and SNAI2 inhibited miR-92b expression (A) The expression of miR-92b-3p in HK2 cells treated with TGF-β for 12 h, as detected by qPCR, n = 5. (B) Sequence logo of transcription factor SNAI1 and SNAI2. (C–E) Protein levels of SNAI1, SNAI2, and β-actin in HK2 cells treated with SNAI1 siRNA (siSNAI1), SNAI2 siRNA (siSNAI2), or control siRNA as indicated in the figure, as detected by western blotting; quantitative results are shown; n = 3. Expression of Col1a1 and miR-92b-3p as determined by qPCR; n = 5. (F) Schematic representation of the human THBS3-AS1 promoters with the putative E2-box promoter shown as a red box. (G) A series of truncated THBS3-AS1 promoters fused to the luciferase reporter gene was co-transfected into HEK293T cells under TGF-β stimulation together with the pcDNA3.1 Renilla plasmid (Ctrl). The relative luciferase activity was presented as folds of Ctrl (n = 3). (H) DNA fragments containing the flanking region of the E2-box on the THBS3-AS1 promoters were immunoprecipitated with anti-SNAI1 or anti-SNAI2 antibodies. Data are expressed as means ± SD; n = 6 per group; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Similar results from three independent experiments are shown.

    Article Snippet: SNAI2 siRNA , Santa Cruz biotechnology , Cat# sc-38393.

    Techniques: Expressing, Sequencing, Control, Western Blot, Luciferase, Transfection, Plasmid Preparation, Activity Assay, Immunoprecipitation

    Journal: iScience

    Article Title: MircroRNA-92b as a negative regulator of the TGF-β signaling by targeting the type I receptor

    doi: 10.1016/j.isci.2023.108131

    Figure Lengend Snippet:

    Article Snippet: SNAI2 siRNA , Santa Cruz biotechnology , Cat# sc-38393.

    Techniques: Recombinant, Control, Plasmid Preparation, Software, Modification, Staining, Extraction, Bicinchoninic Acid Protein Assay, Western Blot, cDNA Synthesis, SYBR Green Assay, Immunohistochemistry, Isolation

    (A) Representative images of primary epicardial cells transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18) or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.

    Journal: PLoS ONE

    Article Title: The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells

    doi: 10.1371/journal.pone.0057829

    Figure Lengend Snippet: (A) Representative images of primary epicardial cells transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18) or Wt1 (siWt1). Scale bar: 200 µm. (B) Immunostaining for ZO-1 or N-cadherin (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. Scale bars: 100 µm. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on the cellular morphology of primary epicardial cells transfected with siRNAs. (D) Representative images of primary epicardial cells transfected with siRNAs at 0 and 14 hr after the scratch was made. Scale bar: 200 µm. (E) Quantification of migration distance, given as a ratio to the siControl (n = 4; * P <0.01 vs. siControl). (F) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the siControl. (G) Western blot performed with antibodies against Tbx18, Wt1, adhesion molecules (E-cadherin and N-cadherin) and EMT regulators (Snail and Slug). β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD.

    Article Snippet: The following siRNAs were used: Tbx18 siRNA (siTbx18; MSS233533, siTbx18-2; MSS233532, Invitrogen), Wt1 siRNA (siWt1; MSS212627, siWt1-2; MSS212628, Invitrogen), Slug siRNA (siSlug; MSS237944, Invitrogen) and control siRNA (siControl; SIC-001, Sigma-Aldrich).

    Techniques: Transfection, Immunostaining, Staining, Migration, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    (A) Representative images of epicardial cells treated with TGFβ1 and transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18), Slug (siSlug) or Wt1 (siWt1). (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis in TGFβ1-treated epicardial cells transfected with siControl, siTbx18, siSlug or siWt1 (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the TGFβ1+siControl. (E) Western blot performed with antibodies against Tbx18, Wt1, Snail and Slug. β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Journal: PLoS ONE

    Article Title: The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells

    doi: 10.1371/journal.pone.0057829

    Figure Lengend Snippet: (A) Representative images of epicardial cells treated with TGFβ1 and transfected with control siRNA (siControl) or siRNA directed against Tbx18 (siTbx18), Slug (siSlug) or Wt1 (siWt1). (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNAs. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 , Snail and Slug by real-time PCR analysis in TGFβ1-treated epicardial cells transfected with siControl, siTbx18, siSlug or siWt1 (n = 3; * P <0.05 vs. siControl). The results were normalized to Gapdh expression, and the relative expression level is given as a ratio to the TGFβ1+siControl. (E) Western blot performed with antibodies against Tbx18, Wt1, Snail and Slug. β-actin and histone H3 were used as loading controls. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Article Snippet: The following siRNAs were used: Tbx18 siRNA (siTbx18; MSS233533, siTbx18-2; MSS233532, Invitrogen), Wt1 siRNA (siWt1; MSS212627, siWt1-2; MSS212628, Invitrogen), Slug siRNA (siSlug; MSS237944, Invitrogen) and control siRNA (siControl; SIC-001, Sigma-Aldrich).

    Techniques: Transfection, Immunostaining, Staining, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    (A) Representative images of Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug. Epicardial cells transfected with siControl or siWt1 were used as controls. (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNA. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 and Slug by real-time PCR in Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug, in comparison to siControl-transfected epicardial cells (n = 3; * P <0.05 vs. siControl, † P <0.05 vs. siWt1). The results were normalized to Gapdh expression and the relative expression level is provided as a ratio to the siControl. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Journal: PLoS ONE

    Article Title: The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells

    doi: 10.1371/journal.pone.0057829

    Figure Lengend Snippet: (A) Representative images of Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug. Epicardial cells transfected with siControl or siWt1 were used as controls. (B) Immunostaining for ZO-1 (green) and DAPI nuclear staining (blue) of primary epicardial cells transfected with siRNA. (C) Percentage of cells categorized as “Enlarged” or “Cobblestone-like,” based on cellular morphology. (D) The relative mRNA expression of Tbx18 , Wt1 and Slug by real-time PCR in Wt1-knockdown epicardial cells co-transfected with siTbx18 or siSlug, in comparison to siControl-transfected epicardial cells (n = 3; * P <0.05 vs. siControl, † P <0.05 vs. siWt1). The results were normalized to Gapdh expression and the relative expression level is provided as a ratio to the siControl. The data are presented as the mean ± SD. Scale bars: 100 µm.

    Article Snippet: The following siRNAs were used: Tbx18 siRNA (siTbx18; MSS233533, siTbx18-2; MSS233532, Invitrogen), Wt1 siRNA (siWt1; MSS212627, siWt1-2; MSS212628, Invitrogen), Slug siRNA (siSlug; MSS237944, Invitrogen) and control siRNA (siControl; SIC-001, Sigma-Aldrich).

    Techniques: Transfection, Immunostaining, Staining, Expressing, Real-time Polymerase Chain Reaction